| Division | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| # | Northeast Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Crown Legacy | 14 | 9 | 4 | 1 | 0 | 29:16 | 36 | |
| 2. | New England Revolution II | 14 | 8 | 4 | 1 | 1 | 32:15 | 33 | |
| 3. | New York Red Bulls 2 | 14 | 8 | 0 | 1 | 5 | 29:23 | 25 | |
| 4. | New York City II | 14 | 7 | 1 | 1 | 5 | 33:25 | 24 | |
| 5. | Philadelphia Union II | 14 | 6 | 1 | 0 | 7 | 26:31 | 20 | |
| 6. | Toronto FC II | 14 | 4 | 2 | 1 | 7 | 22:23 | 17 | |
| # | Central Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Orlando City B | 14 | 9 | 1 | 1 | 3 | 37:29 | 30 | |
| 2. | Columbus Crew 2 | 14 | 9 | 1 | 0 | 4 | 34:28 | 29 | |
| 3. | Atlanta United 2 | 14 | 8 | 1 | 1 | 4 | 32:20 | 27 | |
| 4. | Huntsville | 14 | 6 | 1 | 3 | 4 | 31:22 | 23 | |
| 5. | Chicago Fire II | 14 | 4 | 3 | 4 | 3 | 15:12 | 22 | |
| 6. | Cincinnati 2 | 14 | 6 | 1 | 1 | 6 | 23:28 | 21 | |
| 7. | Inter Miami II | 14 | 4 | 1 | 2 | 7 | 21:29 | 16 | |
| # | Pacific Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Tacoma Defiance | 14 | 9 | 3 | 0 | 2 | 34:18 | 33 | |
| 2. | Portland Timbers 2 | 14 | 8 | 0 | 1 | 5 | 29:18 | 25 | |
| 3. | Vancouver Whitecaps 2 | 14 | 5 | 2 | 1 | 6 | 19:21 | 20 | |
| 4. | Town FC | 14 | 3 | 4 | 1 | 6 | 16:19 | 18 | |
| 5. | Ventura County | 14 | 3 | 4 | 0 | 7 | 16:28 | 17 | |
| 6. | Los Angeles FC II | 14 | 4 | 0 | 3 | 7 | 17:14 | 15 | |
| 7. | Real Monarchs | 14 | 2 | 2 | 2 | 8 | 17:32 | 12 | |
| # | Frontier Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Colorado Rapids 2 | 14 | 9 | 2 | 1 | 2 | 39:20 | 32 | |
| 2. | Austin FC II | 14 | 9 | 2 | 0 | 3 | 28:9 | 31 | |
| 3. | Sporting Kansas City II | 14 | 7 | 4 | 0 | 3 | 32:18 | 29 | |
| 4. | St. Louis City 2 | 14 | 6 | 3 | 1 | 4 | 28:19 | 25 | |
| 5. | Minnesota 2 | 14 | 6 | 3 | 0 | 5 | 26:26 | 24 | |
| 6. | North Texas | 14 | 5 | 1 | 3 | 5 | 29:24 | 20 | |
| 7. | Houston Dynamo 2 | 14 | 4 | 2 | 1 | 7 | 17:21 | 17 | |
| # | Eastern Conference | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Crown Legacy | 14 | 9 | 4 | 1 | 0 | 29:16 | 36 | |
| 2. | New England Revolution II | 14 | 8 | 4 | 1 | 1 | 32:15 | 33 | |
| 3. | Orlando City B | 14 | 9 | 1 | 1 | 3 | 37:29 | 30 | |
| 4. | Columbus Crew 2 | 14 | 9 | 1 | 0 | 4 | 34:28 | 29 | |
| 5. | Atlanta United 2 | 14 | 8 | 1 | 1 | 4 | 32:20 | 27 | |
| 6. | New York Red Bulls 2 | 14 | 8 | 0 | 1 | 5 | 29:23 | 25 | |
| 7. | New York City II | 14 | 7 | 1 | 1 | 5 | 33:25 | 24 | |
| 8. | Huntsville | 14 | 6 | 1 | 3 | 4 | 31:22 | 23 | |
| 9. | Chicago Fire II | 14 | 4 | 3 | 4 | 3 | 15:12 | 22 | |
| 10. | Cincinnati 2 | 14 | 6 | 1 | 1 | 6 | 23:28 | 21 | |
| 11. | Philadelphia Union II | 14 | 6 | 1 | 0 | 7 | 26:31 | 20 | |
| 12. | Toronto FC II | 14 | 4 | 2 | 1 | 7 | 22:23 | 17 | |
| 13. | Inter Miami II | 14 | 4 | 1 | 2 | 7 | 21:29 | 16 | |
| # | Western Conference | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Tacoma Defiance | 14 | 9 | 3 | 0 | 2 | 34:18 | 33 | |
| 2. | Colorado Rapids 2 | 14 | 9 | 2 | 1 | 2 | 39:20 | 32 | |
| 3. | Austin FC II | 14 | 9 | 2 | 0 | 3 | 28:9 | 31 | |
| 4. | Sporting Kansas City II | 14 | 7 | 4 | 0 | 3 | 32:18 | 29 | |
| 5. | St. Louis City 2 | 14 | 6 | 3 | 1 | 4 | 28:19 | 25 | |
| 6. | Portland Timbers 2 | 14 | 8 | 0 | 1 | 5 | 29:18 | 25 | |
| 7. | Minnesota 2 | 14 | 6 | 3 | 0 | 5 | 26:26 | 24 | |
| 8. | Vancouver Whitecaps 2 | 14 | 5 | 2 | 1 | 6 | 19:21 | 20 | |
| 9. | North Texas | 14 | 5 | 1 | 3 | 5 | 29:24 | 20 | |
| 10. | Town FC | 14 | 3 | 4 | 1 | 6 | 16:19 | 18 | |
| 11. | Ventura County | 14 | 3 | 4 | 0 | 7 | 16:28 | 17 | |
| 12. | Houston Dynamo 2 | 14 | 4 | 2 | 1 | 7 | 17:21 | 17 | |
| 13. | Los Angeles FC II | 14 | 4 | 0 | 3 | 7 | 17:14 | 15 | |
| 14. | Real Monarchs | 14 | 2 | 2 | 2 | 8 | 17:32 | 12 | |